FISH- Regenerative Medicine

The field of regenerative medicine involves using human cells and tissues to restore normal function to the body. While bone marrow and skin transplants have been widely used for some time, the field is expanding rapidly. Today, biologics such as stem cells and platelets are being examined for their efficacy in treating everything from arthritis to cancer. While there is much promise in this field for the treatment of disease, there are major ethical concerns, particularly in regards to embryonic and fetal derived stem cells.

The Regenerative Medicine Initiative (RMI) of FISH is focused on fostering collaborative efforts between academic and clinical innovators in the field of regenerative medicine to develop and promote ethically sound therapies to treat disease.

The mission of the RMI is four-fold:

  1. To promote ethically sound regenerative medicine treatments that are consistent with the Church’s teaching and that will promote the culture of life;
  2. To perform basic research aimed at better understanding how to harness the regenerative properties of adult stem cells and other biologics; and
  3. To create opportunities for undergraduate students at Franciscan University of Steubenville to participate in cutting-edge research that has the potential to lead to clinical applications in this exciting field.
  4. To provide laboratory services for clinicians in the field of regenerative medicine.
Regenerative Medicine Lab Analysis Services

In an effort to aid in the progress of regenerative treatments using autologous bone marrow aspirates and autologous fat graphs, Franciscan University’s Regenerative Medicine Lab Analysis Service offers sample analysis to assess the quality of biologics being used for therapeutic purposes. While we provide a variety of laboratory services, we primarily test bone marrow and adipose tissue samples.

Standard Analysis

Nucleated cell count: Using a live/dye fluorescent dye protocol, we determine the live nucleated cell count and the percent viability of the nucleated cells in your sample.

Bone Marrow: $75 per sample
Adipose Tissue: $225 per sample

Sample Analysis Submission Form

CFU-f count: Using a cell culture system we determine the number of fibroblast colony forming units (CFU-f) in your sample. CFU-f numbers are correlated with the number of mesenchymal stem cells in a sample.

Bone Marrow: $225 per sample
Adipose Tissue: $225 per sample

Sampe Analysis Submission Form

Advanced Analysis

Flow cytometry analysis: Using antibodies for specific cell surface markers we are able to perform flow cytometry analysis on both bone marrow and adipose tissue. These include CD271, CD34, and CD 105 cell surface markers. Other surface markers can be analyzed upon request. Pricing varies depending upon the specific markers and the number of samples. Please use the Sample Analysis Inquiry Form to obtain additional information or to request a quote for a specific analysis.

Other laboratory services: In addition to the services described above, we are also able to provide a variety of additional laboratory services to meet your regenerative medicine research needs. Please use the Sample Analysis Inquiry Form to describe your request. We will respond to your inquiry within two business days.

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Regenerative Medicine Research
About Dr. Doroski
Dr. Doroski

Dr. Derek Doroski, assistant professor of Biology, has two major branches to his regenerative medicine research: the chemical combination that could cause adult stem cells to become tendon/ligament tissue; and a “scaffolding” that could hold stem cells near a wound to serve as a “cellular bandage.”

He received his PhD in biomedical engineering from one of the top 3 programs in the nation. During his PhD work, Dr. Doroski explored methods of tendon and ligament regeneration through the use of adult stem cells. This work involved promoting the stem cells to develop by culturing them in a three-dimensional culture environment and exposing the cells to forces similar to the forces experienced in tendon and ligament tissues. Dr. Doroski is now heading up adult stem cell research in the field of Regenerative Medicine with Franciscan students with two new projects: stem cell differentiation and the stem cell “band-aid”.

More About Dr. Doroski
Areas of Research

Stem Cell Differentiation

Mesenchymal stem cells (MSCs) are one of the most commonly studied cells in regenerative medicine. MSCs can become muscle, cartilage, fat, bone, and tendon/ligament cells. Chemical mixes currently exist that can push MSCs toward many of these individual lineages. However, no chemical method for inducing MSCs toward a tendon/ligament cell lineage currently exists. The lack of a tendon/ligament chemical mix is an obstacle to the usage of MSCs for tendon/ligament repair and regeneration therapies. This project has identified a candidate growth factor that is hypothesized to play a significant role in promoting MSCs to become tendon/ligament cells. Further work will seek to confirm the efficacy of this growth factor.

Stem Cell “band-aid”

Stem cells are a major part of tissue engineering approaches for regeneration of damaged tissues and organs. One challenge of stem cell therapies is successfully delivering cells to the wound site. Direct injection of stem cells may result in their diffusion away from the injury site. A biomaterial scaffold containing stem cells can prevent diffusion away from the injury site, but stem cells may be hindered from exiting the scaffold. This project involves investigation of a “cellular patch.” The patch would be applied over a wound site thereby constraining cells over the wound. Over time stem cells will release from the patch and adhere to the wound site. In short, this patch will be designed to act like a “cellular band aid”.

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Support the research our students are doing through FISH by giving to the Science Fund.